Species: Mouse Immunogen: Human breast carcinoma Clone: T2H5 Isotype: : IgG1, kappa Species Reactivity: Human. Others not known. Positive Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell, and lung carcinomas. Staining of normal fibroblasts serves as internal positive control. Specificity: In Western blotting, it reacts with two bands at ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this monoclonal antibody is validated by sequential immunoprecipitation with a polyclonal antibody against Tenascin C.
Species: Mouse Clone: 2H11 Isotype: IgG1, Kappa Species Reactivity: Human Cellular Localization: Cytoplasmic Specificity: Thyroglobulin is a 660 kDa dimeric preprotein with multiple glycosylation sites is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimmers, thyroglobulin monomers undergo conformation maturation in the endoplasmic reticulation. Thyroglobulin dimerization as well as transport of thyroglobulin to the Golgi complex is calcium dependent. Thyroglobulin defects resulting from defective dimer formation and export to the Golgi is thought to cause some types of goiter. Antibody against thyroglobulin may be produced by individuals with other diseases arising from the gland such as Hashimoto's or Graves disease. Hence the presence of thyroglobulin autoantibodies can help to identify disease. Antibody to thyroglobulin has been shown to be useful for the identification of papillary and follicular thyroid carcinoma; thyroglobulin antibody positive lesions are of thyroidal origin. Carcinomas of nonthyroidal origin do not express thyroglobulin and hence are thyroglobulin antibody negative. It is important to note though that not every type of thyroidal lesion is thyroglobulin antibody positive, a number of forms are negative. Hence a negative result does not necessarily rule out that a given lesion or metastasis originated from the thyroid gland.
Species: Mouse Clone: 2H11 Isotype: IgG1, Kappa Species Reactivity: Human Cellular Localization: Cytoplasmic Specificity: Thyroglobulin is a 660 kDa dimeric preprotein with multiple glycosylation sites is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimmers, thyroglobulin monomers undergo conformation maturation in the endoplasmic reticulation. Thyroglobulin dimerization as well as transport of thyroglobulin to the Golgi complex is calcium dependent. Thyroglobulin defects resulting from defective dimer formation and export to the Golgi is thought to cause some types of goiter. Antibody against thyroglobulin may be produced by individuals with other diseases arising from the gland such as Hashimoto's or Graves disease. Hence the presence of thyroglobulin autoantibodies can help to identify disease. Antibody to thyroglobulin has been shown to be useful for the identification of papillary and follicular thyroid carcinoma; thyroglobulin antibody positive lesions are of thyroidal origin. Carcinomas of nonthyroidal origin do not express thyroglobulin and hence are thyroglobulin antibody negative. It is important to note though that not every type of thyroidal lesion is thyroglobulin antibody positive, a number of forms are negative. Hence a negative result does not necessarily rule out that a given lesion or metastasis originated from the thyroid gland.
Species: Mouse Immunogen: Recombinant human ACP5 protein Clone: ACP5/1070 Isotype: IgG2b, kappa Species Reactivity: Human, Mouse, and Rat. Others not known. Positive Control: HepG2, 293T, K562 or RPMI-8226 Cells. Spleen from Hairy Cell Leukemia (HCL) patient. Specificity: This antibody recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This antibody reacts with both the isoforms. The anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
Species: Mouse Immunogen: Recombinant human TEM8 protein Clone: TEM8/589 Isotype: IgG1, kappa Species Reactivity: Human, Non-human Primates, Dog, and Rabbit. Others not known. Positive Control: P23 cells. Colon or breast carcinoma. Specificity: This antibody recognizes a protein of ~63kDa, identified as Tumor Endothelial Marker 8 (TEM8).
Species: Mouse Clone: T311 Isotype: Mouse IgG1 Species Reactivity: Human. Positive Control: Human Melanoma Specificity: Tyrosinase has been shown to be a very specific marker for melanomas, not cross reacting with other tumors or normal tissues tested.
Species: Mouse Clone: T311 Isotype: Mouse IgG1 Species Reactivity: Human. Positive Control: Human Melanoma Specificity: Tyrosinase has been shown to be a very specific marker for melanomas. Cross reactivity with other tumors or normal tissues tested has not been reported.
Species: Mouse Clone: 31A3 Isotype: Mouse IgG1, Kappa Species Reactivity: Cow, Human, Mouse, Pig, Rat. Positive Control: Brain. Specificity: The UchL1 clone 31A3 antibody stains neuronal cell bodies and axons in the central and peripheral nervous systems as well as small nerve fibers in peripheral tissues, including epidermal tissues (Day & Thompson, 2010). The antibody also stains neuroendrocrine cells in the kidney, pituitary, thyroid, pancreas, gastrointestinal tract, and tumors of the diffuse neuroendocrine system. This antibody has also identified UchL1 expression in renal tubule spermatogonia, testis, ovary, and both pregnant and non-pregnant corpus luteum.
Species: Mouse Clone: 31A3 Isotype: Mouse IgG1, Kappa Species Reactivity: Cow, Human, Mouse, Pig, Rat. Positive Control: Brain. Specificity: The UchL1 clone 31A3 antibody stains neuronal cell bodies and axons in the central and peripheral nervous systems as well as small nerve fibers in peripheral tissues, including epidermal tissues (Day & Thompson, 2010). The antibody also stains neuroendrocrine cells in the kidney, pituitary, thyroid, pancreas, gastrointestinal tract, and tumors of the diffuse neuroendocrine system. This antibody has also identified UchL1 expression in renal tubule spermatogonia, testis, ovary, and both pregnant and non-pregnant corpus luteum.
Species: Rabbit Immunogen: Recombinant protein encoding human vimentin. Mol. Weight: 57-60kDa Clone: SP20 Isotype: IgG Specificity: This antibody selectively stains vimentin in human tissue sections. Vimentin is the main intermediate filament in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms. Species Reactivity: Human. Others not tested.
Species: Mouse Monoclonal Clone: V9 Isotype: IgG1/k MW: 57-60 kD Species Reactivity: Human, Monkey, Cow, Horse, Pig, Rabbit, Dog, Cat, Rat, Mouse, Hamster, Gerbil and Chicken. Positive Control: Sarcomas, meningiomas, or melanomas. Specificity: Recognizes a 57-60kDa protein which is identified as vimentin. It shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP.
Species: Mouse Immunogen: Human vimentin recombinant protein Clone: VM452 Isotype: IgG1 Species Reactivity: Human, Cow, Dog, Cat, Pig, Goat and Chicken. Does not react with Mouse and Rat. Others not known. Positive Control: Jurkat cells, Sarcomas, Melanomas. Specificity: This antibody reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.