The Calcium Stain Kit (Modified Von Kossa) is intended for use in the histological visualization of calcium deposits in paraffin or frozen sections. The Modified Von Kossa method is a precipitation reaction in which silver ions react with phosphate (not calcium) in the presence of acidic material. Photochemical degradation of silver phosphate to silver then occurs under UV light illumination. Tissues are treated with a silver nitrate solution and silver replaces calcium reduced by strong light, and so can be visualized as metallic silver. To confirm the presence of calcium, Nuclear Fast Red solution is used to confirm the presence of calcium deposits.
Calcium in mass deposits: Black
Calcium in dispersed deposits: Gray
Cytoplasm: Light Pink
Instructions For Use
Our kit is designed for staining myelin/myelinated axons and Nissil substance on formalin fixed, paraffin-embedded tissue as well as frozen tissue. Our kit is used for identifying the basic neuronal structure in brain or spinal cord sections, and contains a Cresyl Echt Violet counterstain. Using our protocol and the modified formulation, myelinated tissue can be stained in as little as 30 minutes.
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