In Western blotting, unbound membrane sites need to be hidden by blocking agents, in order to prevent non-specific antibody binding that can lead to high background signal. Many laboratories rely upon milk-based protein blockers, often made with powdered milk; batch-to-batch variations in powdered milk within suppliers, and between supplies, can negatively impact western blot quality. In addition, milk can leave a thick layer of proteins that results in non-specific interactions with antibodies, leading to an increased background, and may be incompatible with detection of protein phosphorylation due to the presence of phosphoproteins in milk.IncrediBlock Blocking Agents are manufactured using Good Laboratory Practice to minimize lot-to-lot variance and ensure consistent performance. Our IncrediBlock blocking agents are background cancelling reagents that can be used with a variety of western blotting detection methods, such as chemiluminescence, fluorescent, and phosphoprotein assays.
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