Which Blocking Agent Is Right For You?



In Western blotting, unbound membrane sites need to be hidden by blocking agents, in order to prevent non-specific antibody binding that can lead to high background signal. Many laboratories rely upon milk-based protein blockers, often made with powdered milk; batch-to-batch variations in powdered milk within suppliers, and between supplies, can negatively impact western blot quality. In addition, milk can leave a thick layer of proteins that results in non-specific interactions with antibodies, leading to an increased background, and may be incompatible with detection of protein phosphorylation due to the presence of phosphoproteins in milk.

IncrediBlock Blocking Agents are manufactured using Good Laboratory Practice to minimize lot-to-lot variance and ensure consistent performance. Our IncrediBlock blocking agents are background cancelling reagents that can be used with a variety of western blotting detection methods, such as chemiluminescence, fluorescent, and phosphoprotein assays.

Incrediblock Basic has been developed to use with immunolabeling techniques for the reduction of nonspecific background staining and elimination of serum as a blocking agent; this agent is highly effective need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. This solution has been shown to be effective for immunohistochemical, ELISA, blot and In-situ techniques and requires no mixing or diluting. Incrediblock Advance is a serum-free protein block for immunochemistry; it is widely compatible with a variety of antibodies and more effective at reducing non-specific background staining than normal serum. This product can be used in antibody-based applications where there is a need for background reduction such as Immunohistochemistry, ELISA plate blocking, and western blotting. Incrediblock Advance Free is our unique serum-free protein block for antibody-based applications. Incrediblock Advance Free eliminates the need for matching species with the link antibody and is often, more effective at reducing non-specific background staining than normal serum.