Our Blue Feulgen DNA Ploidy Analysis Staining Kit is a histochemical reagent kit for quantifying the nuclear DNA content in cells. The kit can be used on a variety of specimen types from cytocentrifuge preparations to formalin fixed paraffin embedded tissue sections. A unique formulation eliminates dye precipitation and results in a cleaner stain than conventional Feulgen reagent-based kits.
This stain kit is optimized for DNA Ploidy specimens that will be stained then scanned and viewed on image analysis systems. The basis of the Feulgen staining procedure is the use of an acid hydrolysis step to remove purines from the deoxyribose backbone of the DNA molecule. The newly exposed deoxyribose sugars have an aldehyde group, and this can then be identified by staining with a Schiff reagent (in this case, an aqueous solution of cresyl-violet and sulfurous acid used to test for the presence of aldehydes). The amount of stain color developed is directly proportional to the amount of DNA present in the stained cells.
Store at 2-8 degree C
Our kit is designed for staining myelin/myelinated axons and Nissil substance on formalin fixed, paraffin-embedded tissue as well as frozen tissue. Our kit is used for identifying the basic neuronal structure in brain or spinal cord sections, and contains a Cresyl Echt Violet counterstain. Using our protocol and the modified formulation, myelinated tissue can be stained in as little as 30 minutes.
The Acid Fast Bacteria (AFB) Stain Kit is intended for use in the histological visualization of Acid Fast Bacteria and Tubercle Bacilli. This kit is a rapid 15-minute procedure. The lipoid capsule of the acid-fast organism takes up carbol fuchsin and resists decolorization.
Acid Fast Organisms: Bright Red
Background: Light Green
These Acid Fast Bacteria (AFB) Animal Control Slides contain sections of positive staining goat intestine and negative staining human lung. The positive control slides are intended to be used to verify histological techniques and reagent reactivity, and are not intended to be used for any quantitative purpose.
Tissues are formaldehyde-fixed, paraffin-embedded, heat dried, and cut at 5 microns unless otherwise specified. Standard tissue placement is mid-range of slide.