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Bouin's fixative, developed in 1897, is recommended for general tissue preservation, and may be used as a substitute for Zenker's Fluid. It contains formaldehyde for cytoplasmic fixation, and picric acid for chromatin. It is an especially good agent for preservation of soft tissues and gastrointestinal tract biopsies because this fixative offers better nuclear staining than 10% neutral-buffered formalin.
Our kit is designed for staining myelin/myelinated axons and Nissil substance on formalin fixed, paraffin-embedded tissue as well as frozen tissue. Our kit is used for identifying the basic neuronal structure in brain or spinal cord sections, and contains a Cresyl Echt Violet counterstain. Using our protocol and the modified formulation, myelinated tissue can be stained in as little as 30 minutes.
The Acid Fast Bacteria (AFB) Stain Kit is intended for use in the histological visualization of Acid Fast Bacteria and Tubercle Bacilli. This kit is a rapid 15-minute procedure. The lipoid capsule of the acid-fast organism takes up carbol fuchsin and resists decolorization.
Acid Fast Organisms: Bright Red
Background: Light Green
These Acid Fast Bacteria (AFB) Animal Control Slides contain sections of positive staining goat intestine and negative staining human lung. The positive control slides are intended to be used to verify histological techniques and reagent reactivity, and are not intended to be used for any quantitative purpose.
Tissues are formaldehyde-fixed, paraffin-embedded, heat dried, and cut at 5 microns unless otherwise specified. Standard tissue placement is mid-range of slide.