Ep-CAM / CD326 (Clone Ber-EP4)


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0.1 ml / 20 ug

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Species: Mouse
Immunogen: Human breast cancer MCF-7 cells
Clone: Ber-EP4
Isotype: IgG1, kappa
Species Reactivity: Human. Others not known.
Positive Control: HT29 cells or breast tumor.
Specificity: Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues. Of the 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are negative except for the erythromyeloid cell line K562.

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