Our Hematoxylin and Eosin Staining Kit makes it easy to produce reproducible, high quality H&E histology. The kit contains everything needed for Hematoxylin and Eosin staining and is intended for use in histology and cytology applications. Included in this kit is an optimized formulation of Eosin Y that provides the benefits of a traditional alcoholic formula with significant improvements in usability. Advantages of our kit include lower evaporation rates, better color patterns, a reduced tendency to spill over containers, hands, and countertops, and improved surface tension to remain on tissue section. Mayer's Hematoxylin (Lillie's Modification) produces crisp, intense blue nuclei that provides optimal contrast to the Eosin stained cytoplasm.
Comes with 500 ml bottles (each) of hematoxylin, eosin, and bluing reagent solutions, sufficient for hundreds of slides.
Our kit is designed for staining myelin/myelinated axons and Nissil substance on formalin fixed, paraffin-embedded tissue as well as frozen tissue. Our kit is used for identifying the basic neuronal structure in brain or spinal cord sections, and contains a Cresyl Echt Violet counterstain. Using our protocol and the modified formulation, myelinated tissue can be stained in as little as 30 minutes.
The Calcium Stain Kit (Modified Von Kossa) is intended for use in the histological visualization of calcium deposits in paraffin or frozen sections. The Modified Von Kossa method is a precipitation reaction in which silver ions react with phosphate (not calcium) in the presence of acidic material. Photochemical degradation of silver phosphate to silver then occurs under UV light illumination. Tissues are treated with a silver nitrate solution and silver replaces calcium reduced by strong light, and so can be visualized as metallic silver. To confirm the presence of calcium, Nuclear Fast Red solution is used to confirm the presence of calcium deposits.
Calcium in mass deposits: Black
Calcium in dispersed deposits: Gray
Cytoplasm: Light Pink
Instructions For Use
The Amyloid Stain Kit (Congo Red) is intended for use in the histological visualization of amyloid in tissue sections. Examination under a polarizing microscope results in green birefringence of amyloid.
Amyloid: Red to Pink
Erythrocytes: Light Orange
Eosinophil Granules: Orange to Red