Involucrin (Clone SY5)


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0.1 ml / 20 ug

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Species: Mouse
Immunogen: Purified involucrin from human keratinocytes
Clone: SY5
Isotype: IgG1, kappa
Species Reactivity: Human, Gorilla, Owl monkey, Pig, and Dog. Does not react with Mouse. Others not known.
Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Specificity: This antibody recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.

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