The Picro-Sirius Red Stain Kit (For Cardiac Muscle) is intended for use in the histological visualization of thin septa and collagen fibers. This modification of the Picro-Sirius Red stain eliminates the yellow cytoplasmic staining that can obscure thin collagenous septa. Using this procedure easily allows viewing of collagenous septa as thin as 0.2-0.5 microns. The PSR stain may be viewed using standard light microscopy or polarized light resulting in birefringence of the collagen fibers to distinguish between type I and type III. Light Microscopy Collagen: Red Septa: Red Cytoplasm: Colorless to Slightly Yellow Polarized Light Microscopy Type I (Thick fibers): Yellow-Orange Birefringence Type III (Thin fibers): Green Birefringence
Our kit is designed for staining myelin/myelinated axons and Nissil substance on formalin fixed, paraffin-embedded tissue as well as frozen tissue. Our kit is used for identifying the basic neuronal structure in brain or spinal cord sections, and contains a Cresyl Echt Violet counterstain. Using our protocol and the modified formulation, myelinated tissue can be stained in as little as 30 minutes.
The Acid Fast Bacteria (AFB) Stain Kit is intended for use in the histological visualization of Acid Fast Bacteria and Tubercle Bacilli. This kit is a rapid 15-minute procedure. The lipoid capsule of the acid-fast organism takes up carbol fuchsin and resists decolorization.
Acid Fast Organisms: Bright Red
Background: Light Green
These Acid Fast Bacteria (AFB) Animal Control Slides contain sections of positive staining goat intestine and negative staining human lung. The positive control slides are intended to be used to verify histological techniques and reagent reactivity, and are not intended to be used for any quantitative purpose.
Tissues are formaldehyde-fixed, paraffin-embedded, heat dried, and cut at 5 microns unless otherwise specified. Standard tissue placement is mid-range of slide.