Teomics proudly introduces SlideMaster, a humidity chamber designed by a histotechnologist that eliminates the majority of individual slide handling.
SlideMaster has three different parts: a humidity chamber, two individual slide holders with white contrast bars, and a waste receptacle. It has been designed so that all three components are compact and easy to use. A maximum of 20 slides can be used per unit.
To use, a hydrophobic barrier is drawn around the tissue section and slides are put in a special slide holder that secures them into place. Drops of antibody are placed on the slides and incubated. After incubation, the individual slide holders can be tilted and secured at a 45 degree angle. All 20 slides are rinsed off in one easy motion and the buffer drains into the waste receptacle. Slides are gently blotted (tissue paper) at the bottom portion of the hydrophobic barrier, which draws off any remaining liquid on the tissue.
The slide holders are then placed back in the horizontal position and incubation and rinse steps are repeated in the same fashion. For chromogen development, the SlideMaster unit has a contrast bar that changes the background from black to white. The black background produces high contrast so that the tissues mounted glass slides can be easily seen, and the white background produces high contrast and allows the technician to monitor the chromogen development. Slides are then rinsed and counterstained and are then ready for coverslipping. SlideMaster is very easy to use. It is light weight and durable. By making the hydrophobic barrier around the tissue section as small as possible, the total volume of antibody reagent can be significantly reduced per slide. Instead of using 100-200 microliters of antibodies per tissue section usually 50 microliters will completely cover small tissues and 100 microliters will cover larger tissues. With this method, slide staining is much more efficient, more consistent, drying-out of slides is minimized, and the cost of reagents is reduced.
Our kit is designed for staining myelin/myelinated axons and Nissil substance on formalin fixed, paraffin-embedded tissue as well as frozen tissue. Our kit is used for identifying the basic neuronal structure in brain or spinal cord sections, and contains a Cresyl Echt Violet counterstain. Using our protocol and the modified formulation, myelinated tissue can be stained in as little as 30 minutes.
Teomics offers an array of new antigen retrieval products that make immunostaining quick and easy. 4Gone is a unique antigen retrieval system that utilizes gentle heating at low temperatures, allowing you to retrieve a large variety of antigens that can't been seen with other reagents.