This liquid substrate for peroxidase consists of tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase on membranes and other surfaces. When reacted with peroxidase, the result is an insoluble blue reaction product. This reagent is not designed for use in microwell applications.
TWEEN 20 (Polyoxyethylenesorbitan Monolaurate) is a versatile nonionic detergent that is widely incorporated in biochemical and immunohistochemical applications, as well as an agent that can be used to prepare emulsions. In biochemical studies, TWEEN 20 can be used for lysing mammalian cells at concentrations of 0.005 to 0.5% and is useful for removal of peripheral proteins in membrane pre-extraction proceedures. TWEEN 20 is also widely used as a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%, and is often incorporated in immunohistochemical buffers at a concentration of 0.1%.
TWEEN 20 is heat sensitive and should be used only after testing, particularly with autoclaving when metal cations are present in solutions. Tween 20 is generally have been reported to be incompatible with a variety of alkali agents, heavy metal salts, tannic acid and phenols, and may also reduce the activity of many preservatives.
Solutions made with Tween 20 have a limited shelf-life (particularly at room temperature) because polysorbates such as Tween 20 undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.
Teomics' Stop Buffer (TSB) offers a unique combination of acid that produces a more stable stopped reaction product than other formulations of H‚‚SO‚„ or HCl. Stopped reactions show increased absorbance values of approximately two-fold over unstopped reactions with minimal drift for up to six hours depending on various conditions.
TMB - This standard sensitivity liquid substrate for peroxidase consists of Tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase in ELISA systems which is stable for long-term storage providing sensitivity greater than OPD. This reagent is available as a single component, ready-to-use reagent. When used in conjunction with our proprietary TMB Stop Buffer (TSB), the resulting color product will not fade or darken for several hours. Our TMB products have been in production for over twenty years and have built a reputation for robust performance, unparalleled stability and next to zero lot-to-lot variation.